Cloning and sequencing of the porcine interleukin-12 receptor beta-1 and beta-2 chains

ABSTRACT

The genes encoding for the porcine beta-1 and beta-2 chains of the porcine interleukin-12 receptor have been cloned. The complete sequence of complementary DNA of both chains has been determined. Variants have been found associated with superior cellular immunity. Applications of the novel molecules are discussed.

BACKGROUND OF THE INVENTION

[0001] The genetics of the porcine IL-12 receptor are determined.

[0002] The genes encoding the porcine beta-1 and beta-2 chains of the porcine interleukin-12 receptor were cloned. The complete sequence of the complementary DNA of both chains was determined. Variants (polymorphisms) were identified that are correlated with immune function.

[0003] Interleukin-12 (IL-12) is a heterodimeric cytokine consisting of two subunits having molecular weights of 35 and 40 kDa. Both subunits are highly conserved across species, with the predicted amino acid sequences of the two porcine IL-12 subunits being approximately 85% homologous with their human counterparts (Foss and Murtaugh, 1997). IL-12 is the single major factor that is required for the efficient differentiation of naive T cells into memory/effector T-cells capable of producing IFN-gamma. Also known as an NK cell stimulatory factor, IL-12 is a cytokine with clear pro-inflammatory functions. IL-12 stimulates the proliferation of activated T and NK cells, and also stimulates the lytic activity of both cytotoxic T lymphocytes and NK cells (Romani, et al., 1997; Trinchieri and Scott, 1994; Trinchieri, 1997). The biological activity of IL-12 is mediated via binding to specific cell surface receptors.

[0004] The human IL-12 receptor consists of a disulfide-linked oligomer composed of two beta-type cytokine receptor subunits termed IL-12 receptor beta-1 and IL-12 receptor beta-2 and having an estimated molecular weight of 130 kDa each. Together these two subunits give rise to the high affinity IL-12 receptor, whereas each independently expressed chain exhibits low affinity for IL-12 (Presky et al., 1996, 1998). The expression of both IL-12 receptor beta-1 and IL-12 receptor beta-2 subunits is upregulated following activation in CD4⁺, CD8⁺ lymphocytes as well as in NK cells (Szabo et al., 1997; Wu et al., 1997). However, expression of the two IL-12 receptor subunits appears to be differentially regulated, at least at the transcriptional level. Although the production of mRNA for both chains is induced during the activation of T cells, transcription of the IL-12 receptor beta-2 gene appears to be more tightly regulated, in that only this subunit, but not the beta-1 subunit is inhibited by IL-10 and TGF-beta (Wu et al., 1997). The biological activity of IL-12 is dependent on the cell surface expression of the receptors for this cytokine. Allelic variants of this beta-1 receptor have been shown to affect the ability of humans to develop a protective immunity against intracellular pathogens such as Salmonella and Mycobacteria.

[0005] Interleukin-12 pays a major role in determining the nature of the immunity that develops in response to infection or vaccination against an infectious agent. This effect is mediated through the binding of this cytokine to its cell surface receptor on lymphoid cells.

[0006] If genetic variants of IL-12 receptors exist in other species, selective breeding could improve animal herd health. Because IL-12 plays a key role in regulating the development of cell-mediated immunity, it is possible that within the swine population there are animals that possess IL-12 receptor alleles that have a superior ability to respond to the differentiation signals provided by IL-12. These animals may consequently be genetically predisposed to develop a strong cellular immune response upon infection or vaccination against a pathogen. Superior cellular immune response is likely to provide a higher level of protective immunity against infectious diseases in where this type of immunity plays a role in protection from disease. What is needed is to develop a genetic marker that will identify animals such as swine capable of developing a strong cellular immune response to a given pathogen.

SUMMARY OF THE INVENTION

[0007] The present invention relates genetic markers that can identify swine with superior cellular immunity. The markers are allelic variants of the IL-12 receptor gene, which consists of 2 subunits.

[0008] An aspect of the invention is a porcine IL-12 receptor encoded by the cDNA molecules disclosed herein. A cDNA molecule encoding the porcine beta-1 chain of the porcine interleukin-12 receptor has a nucleotide sequence as shown in FIGS. 1 and 2. A molecule with an amino acid sequence deduced from the cDNA sequence of the beta-1 chain is also shown in FIGS. 1 and 2. A variant of a cDNA molecule encoding the porcine beta-1 chain of the porcine interleukin-12 receptor which lacks 37 nucleotides from positions 628-664 and results in a premature termination of the IL-12R beta portion as shown in FIGS. 3 and 4. A molecule with an amino acid sequence deduced from the cDNA sequence is also shown in FIGS. 5 and 6. A cDNA molecule encoding the porcine beta-2 chain of the porcine interleukin-12 receptor has a nucleotide sequence as shown in FIGS. 5 and 6. A molecule with an amino acid sequence deduced from the cDNA sequence is also shown in FIGS. 5 and 6. This molecule is present in pigs found to have a high IFN-g response or cell mediated immunity to an infectious agent.

[0009] An aspect of the invention is a molecule with an amino acid sequence having an allelic variant at position 1254 that results in a serine at amino acid position #342 as shown in FIG. 5. The variant results from a nucleotide change at position 1254 from adenine to cytosine. The variant is present in pigs found to have a high IFN-gamma response or cell-mediated immunity to an infectious agent PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) in a pig.

[0010] The invention also relates a method of identifying pigs capable of developing a strong cellular immune response to a given pathogen. The method includes the steps of:

[0011] (a) specifying a given pathogen; and

[0012] (b) selecting pigs with allelic variants of an IL-12 receptor that confer on the pigs a superior ability compared to pigs without the variants, to develop a strong cellular response upon infection or vaccination against the pathogen.

[0013] An aspect of the invention is a method for improving the cellular immune response of pig herds. The method includes the following steps:

[0014] (a) selecting pigs with allelic variants of an IL-12 receptor that confer on the pigs a superior ability compared to pigs without the variants, to develop a strong cellular response upon infection or vaccination against a pathogen; and

[0015] (b) breeding the selected pigs according to methods known to those of skill in the art of pig breeding to produce pigs with improved cellular immunity.

BRIEF DESCRIPTION OF THE DRAWINGS

[0016]FIG. 1 shows the cDNA sequence (SEQ ID NO: 7) for the pig IL-12R beta-1 chain. The nucleotide sequence is on the bottom with the deduced amino acid sequence (SEQ ID NO: 8) on top. A variant of the IL-12 receptor beta-1 chain that results in a premature termination of the coding sequence and a truncated protein has a deletion (underlined). The resulting sequence follows in order the full sequence of the beta-1 chain. A portion of a gene is deleted in the variant resulting in a stop codon downstream. This results in premature termination of protein translation.

[0017]FIG. 2 is the nucleotide sequence of FIG. 1 (SEQ ID NO: 7) in a more compact form without the amino acid sequence on top.

[0018]FIG. 3 is a variant that lacks 37 nucleotides from positions 628-664 in the pig IL-12R beta-1 cDNA (SEQ ID NOS: 9 and 10). The deletion results in premature termination of the IL-12R beta-1 protein. The nucleotide sequence of the variant extends from positions 58-2318 in FIG. 1 in the pig IL-12R beta-1 cDNA (position 1 in FIG. 3 is position 58 in FIG. 1).

[0019]FIG. 4 is FIG. 3 (SEQ ID NO: 9) without the amino acids corresponding to the nucleotide code.

[0020]FIG. 5 shows the amino acid and corresponding nucleotide sequence for pig IL-12R beta-2 cDNA (SEQ ID NOS: 11 and 12). A variation on the beta-2 chain changes the amino acid sequence from a tyrosine to a serine. There is a mutation (polymorphism) from A→C (underlined) at position #1254 that changes the amino acid encoded from tyrosine to serine. The change results in a non-conservative replacement of tyrosine by serine which causes an increased response to infectious agents.

[0021]FIG. 6 is FIG. 5 (SEQ ID NO: 11) showing only the nucleotide sequence.

[0022]FIG. 7 shows the intensity of the cellular immune response of swine following immunization with a PRRSV MLV vaccine. Twenty-four full-sibling pigs (shown in Table 1) were immunized at 9 and 12 weeks of age with a PRRSV MLV vaccine. Peripheral blood mononuclear cells were isolated from them at 2 weeks after the second immunization and stimulated with PRRSV virus for 20 hours. The frequency of IFN-gamma secreting cells was determined with an IFN-gamma ELISPOT assay as described in the Materials and Methods section herein (Zuckermann et al., 1988). The results obtained were separated into two groups according to the genotype of the responder pig into IL-12R beta-2^(c/c) (8 pigs), IL-12R beta-2^(a/c) (9 pigs), IL-12R beta-2^(a/a) (7 pigs). The data for these four weeks was analyzed by ANOVA (Analysis of Variance).

DESCRIPTION OF THE INVENTION

[0023] The present invention relates methods and compositions to identify pigs with superior cell-mediated immunity based on their IL-12R genotype. The receptor and its coding sequences were identified. cDNA sequences and deduced amino acid sequences of the 2 subunits forming porcine IL-12 receptor are disclosed. These cloned porcine genes and predicted immune response of pigs due to IL-12 receptor genotype differences, have not been previously reported.

[0024] Information obtained on the full sequence of both chains of the porcine IL-12 receptor, is useful to identify allelic variants of these genes that differ in their function. Because IL-12 binds to its receptor on the surface of T cells, structural (sequence) variations on the gene may affect the functionality of the receptor. The invention relates to effects of allelic variations on the functionality of the IL-12 receptor which are likely to have an impact on the ability of a pig to develop a cellular immune response to an infectious agent. As discussed in the Background, IL-12 is known to control the development of cell-mediated immunity.

[0025] The cloning and sequencing of the porcine IL-12 receptor is a first step in the identification of animals with superior cellular immunity. Having obtained the sequence of the genes encoding for the two chains of the receptor (FIGS. 1-4, showing the beta-1 chain and FIGS. 5 and 6 showing the beta-2), allelic variants of these genes were identified that allow for a higher level of a cellular immune response to microorganisms as compared to pigs without the variants.

[0026] The IL-12 receptor consists of two chains (subunits), the IL-12R beta-1 and IL-12 beta-2 chains. Each chain was individually cloned and sequenced. FIGS. 1-4 show the DNA sequences of the IL12R beta-1 chain. A variant of the IL-12 receptor beta-1 chain was discovered which results in premature termination of the coding sequence. In this variant, the resulting protein translation terminates prematurely (see FIGS. 1 and 2). The variant of the IL-12R beta-1 chain lacks 37 nucleotides from positions 628 through 664. This deletion results in a premature termination of the IL-12R beta-1 protein.

[0027] To determine the IL-12R beta-2 cDNA sequence, four groups of pigs were obtained from the Pig Improvement Company (PIC). Each group of pigs was sequenced to determine the IL-12R beta-2 cDNA. These sequences were compared and the following polymorphisms were determined: Position 245 (G to T changes: Glycine to Cysteine. Non-conservative replacement. Located in leader sequence); Position 556 (T to G changes: Aspartic acid to Glutamic acid. Conservative replacement); Position 890 (A to G changes: Isoleucing to Valine. Conservative Replacement); Position 1254 (A to C changes: Tyrosine to Serine. Non-conservative replacement); Position 2233 (C to T changes: Aspartic acid to Aspartic acid. No change in identity); and Position 2440 (C to T changes: Histidine to histidine. No change in identity).

[0028] Allelic variation of the IL-12 receptor beta-2 (IL-12RBeta-2) chain of this molecule has an impact on the ability of a pig to develop a cellular immune response to an infectious agent. Of all the variants discovered, a polymorphism at position 1254 (a nucleotide change from A to C) within the porcine IL-12 beta-2 subunit gene) (FIGS. 5 and 6), which results in either a serine or a tyrosine at amino acid position #342 of the intact swine IL-12R beta-2 chain was of particular interest. This change has an impact on the intensity of the cell-mediated immune response to an infectious agent. Only the former amino acid has been detected at the corresponding site in IL-12R beta-2 of either human, murine or bovine origin. The IL-12 beta-2 allele expressing a serine at nucleotide position 1254 in pigs is associated with a greater host interferon-gamma response to immunization with porcine reproductive and respiratory virus (FIG. 7). The expression of this allele is likely associated with a higher interferon gamma response to other microorganisms of swine in general.

[0029] Initially, the virus-specific immunity elicited in a group of pigs in response to vaccination with a modified live virus (MLV) vaccine against porcine reproductive and respiratory syndrome virus (PRRSV) was evaluated. The MLV is a non-virulent attenuated vaccine manufactured by Boehringer Ihgelheim Vet Medica. The PRRSV is a stock arterivirus (strain VR2332 from the American Type Culture Collection). One pig from this injection experiment (designated No. 785g) was unique. In this animal the frequency of PRRSV-specific IFN secreting cells in the peripheral blood elicited in response to immunization with this virus was more than double that of any of the other nine pigs in the group. Thus, this pig appeared to be a high responder to PRRSV antigens. To extend this observation and determine the genetic basis for the high responder phenotype the mother and father of pig 785g were bred and produced 24 full siblings to this original animal. The genotype of these pigs for the IL-12R beta-2 chain at positions 1254 is shown in Table 1. TABLE 1 IL-12R Beta-2 Genotype of Full-Siblings of Pig 785 g. Litter 1 Litter 2 Pig. No. Genotype* Pig. No. Genotype 1 a/c 211 a/a 2 c/c 212 a/a 3 a/a 213 c/c 4 c/c 214 a/c 5 c/c 216 a/c 6 a/a 217 a/c 7 c/c 218 a/c 8 a/c 219 c/c 9 c/c 222 a/a 10 c/c 221 a/c 12 a/c 222 a/c 13 a/a 223 a/a

[0030] Using the pigs described in Table 1 animals expressing a “c” allele for the IL-12R beta-2 were more likely to have the ability to develop a high virus-specific IFN-gamma response following an immunization with a PRRSV modified live virus vaccine than those not expressing this allele. As shown in FIG. 7, the virus-specific IFN-gamma response of pigs with the IL-12R beta-2^(a/c) genotype was significantly higher (p<0.01) than pigs with the IL-12R beta-2^(a/a) genotype at 6 weeks following vaccination. Although the IFN-gamma response of pigs with the IL-12R beta-2^(c/c) genotype was not significantly higher than that of pigs with the IL-12R beta-2^(a/a) genotype, it followed the same high response trend seen with the IL-12R beta-2^(a/c) pigs.

[0031] Materials and Methods

[0032] Method for Differentiation of “A” and “C” Homozygotes and Heterozygotes to Nucleotide Position 1254 of the Sequenced Porcine IL-12R Beta-2 cDNA.

[0033] To identify the polymorphism associated with nucleotide position 1254 of the porcine IL-12R Beta 2 cDNA, primers were engineered to create either one or two novel EcoR I sites in the amplicon generated during amplification of a portion of this template. The forward PCR primer (SEQ ID NO: 1) (GACTACACAAGACAACAGAATT) is nearly identical to the nucleotide stretch (SEQ ID NO: 2) (GACTACAAAAGACAACAGATTT) immediately upstream of the polymorphic site. Use of this primer results in the third upstream thymidine being converted to an adenosine moiety. Thus, when a cytosine is present at the polymorphic site, an EcoR I recognition sequence (GAATTC) is generated. When an adenosine is present, the EcoR I recognition sequence is not made. The reverse PCR primer (SEQ ID NO: 3) (TATTATGCAGTGTGGAATTCCC; complement—(SEQ ID NO: 4) GGGAATTCCACACTGCATAATA) is nearly complementary to a nucleotide stretch (SEQ ID NO: 5) (GGGAATGCCACACTGCAGAATA) downstream of the polymorphic site. Use of this primer results in the conversion of the guanosine at position seven to a thymidine moiety and the creation of an EcoR I recognition sequence.

[0034] Differentiation of the A/C polymorphism is accomplished in the following manner. First, RNA is isolated from swine leukocytes previously activated by exposure to a mitogen such as phytohemmaglutinin. It should be noted that since IL-12R Beta 2 transcripts are not present in detectable levels in quiescent cells, their production needs to be stimulated. Then, the RNA is reverse transcribed by using random hexamers as primers and a portion of the resultant IL-12R Beta 2 cDNAs are amplified by a “proof-reading” polymerase. Amplicons generated by the two above mentioned primers are 127 bp in length and contain either one or two acquired EcoR I recognition sites depending on whether an adenosine or cytosine moiety is present at the polymorphic site, respectively. Digestion of the products with EcoR I yields either a 110 (adenosine) or 90 (cytosine) bp product which can easily be identified based on their relative mobilities in polyacrylamide gels.

[0035] Method for Pig IFN-Gamma Elispot Determination of Cell-Mediated Immunity

[0036] The magnitude of the cellular immune response to immunization with the PRRS virus vaccine was quantified by utilizing an IFN-ELISPOT assay. Briefly, 96-well Immulon II™ plates (Dynatech Inc.) were coated with 50 ml per well of a 24 μg/ml solution of mAb P2G10 in 0.1 M carbonate buffer, pH 9.6. After an overnight incubation at 4° C., each well was washed three times with sterile PBS and then incubated with 50 μl of RPMI supplemented with 5% fetal porcine serum for two hours at 37° C. in a 5% COPeripheral blood mononuclear cells (PBMC) from vaccinated or control pigs were plated at 5×10⁵ viable cells per well. In all samples, PBMC were >98% viable as confirmed by vital dye exclusion. The in vitro recall response to PRRSV was stimulated by the addition of antigen in the form of live virus. PBMC were exposed to viral antigen at 37° C. in a 5% CO by washing the wells six times with phosphate buffered saline (PBS) supplemented with 0.05% Tween-20. Fifty μl of 0.25 μg/ml biotin-labeled mAb P2C11 in 0.05% PBS-Tween was then added to each well and the plates were incubated for 1 hour at 37° C. After washing, 50 μl of 0.31 μg/ml of streptavidin-horseradish peroxidase (SA-HRP; Zymed; San Francisco, Calif.) was added to each well and the plates were incubated for an additional hour at 37° C. Excess SA-HRP was removed by washing the wells 3 times and then 50 μl of TMB membrane peroxidase substrate (Kirkegaard & Perry Laboratories, Gaithersburg, Md.) was added to each well. Hydrolysis of this compound results in the development of blue spots whose size and intensity are directly proportional to the amount of bound IFN-. The frequency of virus-specific IFN-SC was determined by enumerating the blue spots.

[0037] Method for Selecting and Breeding Pigs to Improve Immunity

[0038] In order to select pigs for breeding which express the improved immunity, the pigs will first be screened through the method for differentiation of A and C homozygotes and heterozygotes at nucleotide position 1254 of the sequenced porcine IL-12R Beta-2 cDNA as previously stated in the Materials and Method section. After that screening process has occurred, pigs will be selected which contain the C marker. After selection these pigs will be bred by conventional methods known to those of skill in the art of pig breeding to increase the frequency of the “C” polymorphism in the herd.

[0039] Method of Identifying Polymorphisms in the Porcine IL-12R Subunits

[0040] To identify polymorphisms within the two porcine IL-12R subunit genes, the intact open reading frames encoding these entities were determined by sequencing overlapping RT-PCR products derived from the respective transcripts. Initially, the source of RNAs was from activated T cells obtained from three pigs selected at random from each of the four above mentioned groups of swine provided by POC. For comparison, similar determinations were made for pigs derived from GENETIPORC breeding stock (University of Illinois Veterinary Medicine Swine research Farm). These animals had been participants in an unrelated experiment evaluating immunity elicited in response to vaccination with a PRRS modified live virus (MLV). Within this group of ten, pig 758g was unique in that the frequency of its PRRS virus-specific IFN-gamma secreting cells was more than double that of any of the other nine pigs. Moreover, the novelty of pig 758g's response was even more apparent in view of the fact that immunization with a PRRS MLV vaccine has been shown to elicit a weak cell-mediated immune response. In this regard, the frequency of IFN-gamma secreting cells usually ranges between 50-150 per million mononuclear cells. Thus, based on these values, pig 758g was considered to be a high responder whereas pig 834b for example was considered to be a low responder to recall PRRS virus antigens. Due to the divergence in their immune response phenotypes, the predicted primary structures of the IL-12 receptors were determined.

[0041] Method of Discovering Receptors

[0042] Initially, pairs of degenerate primers, representative of conserved sequences in cDNA encoding the beta-1 and beta-2 subunits of the mouse and human IL-12 were used to amplify internal portions of the respective porcine cDNAs by RT-PCR. Subsequently, the remainder of both cDNAs was obtained by 5′ and 3′ RACE combined with “primer walking.” Both entities have been completely sequenced in both directions. The coding portion of the IL-12R beta-1 cDNA is 2196 nucleotide, while that for IL-12R beta-2 is 2583 nucleotides.

[0043] Both chains of the IL-12R are members of the class I cytokine receptor family which includes gp130, granulocyte colony stimulating factor receptor, and leukemia inhibitory factor receptor. All members of this family share several structural characteristics. Accordingly, both of the porcine IL-12R chains have these characteristics which include a Cys-Cys pair (C/CxW) motif in the amino terminal part of their extracellular domain and a (SEQ ID NO: 6) Trp-Ser-x-Trp-Ser (WS x WS) motif in the C-terminal portion. Presumably, these sequences contribute to ligand interaction and protein architecture. Moreover, the intracellular portion of the porcine IL-12R beta-2 chain contains three tyrosine conserved in the human and mouse counterparts. In these two species, these three tyrosines become phosphorylated upon triggering of the IL-12R and induce activation of quiescent cytoplasmic transcriptional factors. In addition, the intracellular domain of both porcine IL-12R chains has two regions of amino acids, termed box 1 and box 2, which are required fro signal transduction. Box 1 is comprised of a Pro-X-Pro sequence preceded by a cluster of hydrophobic amino acids. The second motif, box 2, has a distinct sequence that is not conserved in all members of the family. The presence of all these structural features demonstrates unequivocally that the identified two porcine IL-12R subunits are indeed members of the superfamily of cytokine receptors.

DOCUMENTS CITED

[0044] Chizzonite R, Truitt T, Desai B B, Nunes P, Podlaski F J, Stern A S, Gately M K. IL-12 receptor. I. Characterization of the receptor on phytohemagglutinin-activated human lymphoblasts. J. Immunol. May 15, 1992;148(10):3117-24.

[0045] Chua A O, Chizzonite R, Desai B B, Truitt T P, Nunes P, Minetti L J, Warrier R R, Presky D H, Levine J F, Gately M K, et al. Expression cloning of a human IL-12 receptor component. A new member of the cytokine receptor superfamily with strong homology to gp130. J. Immunol. Jul. 1, 1994; 153(1):128-36.

[0046] Desai, B. B., Quinn, P. M., Wolitzky, A. G., Mongini, P. K. A., Chizzonite, R., and Gately, M. K. 1992. IL-12 Receptor. II. Distribution and Regulation of Receptor Expression. J. Immunol. 148(10):3125-3132

[0047] Presky D H, Yang H, Minetti L J, Chua A O, Nabavi N, Wu C Y, Gately M K, Gubler U A functional interleukin 12 receptor complex is composed of two Beta-type cytokine receptor subunits. Proc. Natl. Acad. Sci. USA. Nov. 26, 1996;93(24):14002-7.

[0048] Residual type 1 immunity in patients genetically deficient for interleukin-12 receptor Beta-1 (IL-12RBeta-1): evidence for an IL-12RBeta-1-independent pathway of IL-12 responsiveness in human T cells. J. Exp. Med. Aug. 21, 2000;192(4):517-528

[0049] Verhagen C E, de Boer T, Smits H H, Verreck F A, Wierenga E A, Kurimoto M, Lammas D A, Kumararatne D S, Sanal O, Kroon F P, van Dissel J T, Sinigaglia F, Ottenhoff T H.

[0050] [From gene to disease; mutations in interleukin-12-receptor-beta 1- and interferon-gamma-receptor-1 lead to nontuberculous mycobacterial infections and salmonellosis]. Ned Tijdschr Geneeskd Sep. 16, 2000;144(38):1830-2.

[0051] [Article in Dutch] van Dissel J T, Ottenhoff T H. Afd. Infectieziekten, Leids Universitair Medisch Centrum, Leiden.

[0052]

1 12 1 22 DNA Artificial Sequence Description of Artificial Sequence Primer 1 gactacacaa gacaacagaa tt 22 2 22 DNA Artificial Sequence Description of Artificial Sequence Primer 2 gactacaaaa gacaacagat tt 22 3 22 DNA Artificial Sequence Description of Artificial Sequence Primer 3 tattatgcag tgtggaattc cc 22 4 22 DNA Artificial Sequence Description of Artificial Sequence Primer 4 gggaattcca cactgcataa ta 22 5 22 DNA Artificial Sequence Description of Artificial Sequence Primer 5 gggaatgcca cactgcagaa ta 22 6 5 PRT Artificial Sequence Description of Artificial Sequence Synthetic peptide motif 6 Trp Ser Xaa Trp Ser 1 5 7 3135 DNA Porcine sp. CDS (76)..(2271) 7 tgaggactgg ttggctgggg gccttgctgg tagcacagaa gcttgtctgg agctgctgat 60 ctctgcaagg accgg atg tgg caa cga gtg acc agg ctg gtc ccc ctc ttc 111 Met Trp Gln Arg Val Thr Arg Leu Val Pro Leu Phe 1 5 10 ctc ctc ctg ttc ccg agg cag ggt gct gaa gcc tgc agt acc agc agg 159 Leu Leu Leu Phe Pro Arg Gln Gly Ala Glu Ala Cys Ser Thr Ser Arg 15 20 25 tgc tgt ttc cag gac ccg cca tat ccg gat gca gac tca ggc tca gct 207 Cys Cys Phe Gln Asp Pro Pro Tyr Pro Asp Ala Asp Ser Gly Ser Ala 30 35 40 tct ggc ccc agg gac cta aac tgc tac cgg ata ttc aac aga gct ggt 255 Ser Gly Pro Arg Asp Leu Asn Cys Tyr Arg Ile Phe Asn Arg Ala Gly 45 50 55 60 tat gaa tgt tcc tgg gag tat gag ggc ccc aca gct ggg gtc agc cac 303 Tyr Glu Cys Ser Trp Glu Tyr Glu Gly Pro Thr Ala Gly Val Ser His 65 70 75 ttc ctg aga tgc tgc ctc aag tct ggg cgc tgt tgc tac ttt gcc aca 351 Phe Leu Arg Cys Cys Leu Lys Ser Gly Arg Cys Cys Tyr Phe Ala Thr 80 85 90 ggc tca gcc acc aca ctg cag ttt tcc gac cag gat ggc gta gac gtg 399 Gly Ser Ala Thr Thr Leu Gln Phe Ser Asp Gln Asp Gly Val Asp Val 95 100 105 ctc aat gct gtc act ctc tgg gtg gaa tcc cgg gct gcc aac aag acg 447 Leu Asn Ala Val Thr Leu Trp Val Glu Ser Arg Ala Ala Asn Lys Thr 110 115 120 gag aag tcc ccc atg gtt acc ttg aat ctc tac agc tct gtt aaa tac 495 Glu Lys Ser Pro Met Val Thr Leu Asn Leu Tyr Ser Ser Val Lys Tyr 125 130 135 140 gac cct ccc cca gga aac atc aag gta tca agg tca gca ggg cag ctg 543 Asp Pro Pro Pro Gly Asn Ile Lys Val Ser Arg Ser Ala Gly Gln Leu 145 150 155 cac atg gag tgg gag acc cca acc cgc cag gat ggt gct gag ata cag 591 His Met Glu Trp Glu Thr Pro Thr Arg Gln Asp Gly Ala Glu Ile Gln 160 165 170 ttc cga cac cgg aca cct gga agc ccg tgg atg ctg ggc gac tgt gga 639 Phe Arg His Arg Thr Pro Gly Ser Pro Trp Met Leu Gly Asp Cys Gly 175 180 185 cgt cag gat gat gac gct ggc ttc gag tca tgc cta tgc ccc ctg gag 687 Arg Gln Asp Asp Asp Ala Gly Phe Glu Ser Cys Leu Cys Pro Leu Glu 190 195 200 atg gac atg gcc caa gaa ttc cag ctg cga cga cga cga cac cag ggg 735 Met Asp Met Ala Gln Glu Phe Gln Leu Arg Arg Arg Arg His Gln Gly 205 210 215 220 cca ggg gtc cca gga ggc ccc tgg agc agc tgg agc agc tct gtg tgc 783 Pro Gly Val Pro Gly Gly Pro Trp Ser Ser Trp Ser Ser Ser Val Cys 225 230 235 atc ccc cct gaa aac ccc cca cag gcc aag gcg aat ttc tca gtg gag 831 Ile Pro Pro Glu Asn Pro Pro Gln Ala Lys Ala Asn Phe Ser Val Glu 240 245 250 cag ctc cgc tca gat ggg agg agg cag gtg acc ttg aat gag cag ctg 879 Gln Leu Arg Ser Asp Gly Arg Arg Gln Val Thr Leu Asn Glu Gln Leu 255 260 265 ccc cag ctt gag ctt ccg gaa ggc tgc ctt ggg aac aac tct gga gtg 927 Pro Gln Leu Glu Leu Pro Glu Gly Cys Leu Gly Asn Asn Ser Gly Val 270 275 280 gaa atg acc tac cat gtt cac ctg cac atg ctg tcc tgc cct tgt aag 975 Glu Met Thr Tyr His Val His Leu His Met Leu Ser Cys Pro Cys Lys 285 290 295 300 gcc aag gcc aca agg act ctg cgc cta agg aaa aag ctc gtc ctc tca 1023 Ala Lys Ala Thr Arg Thr Leu Arg Leu Arg Lys Lys Leu Val Leu Ser 305 310 315 ggc gct gcc tat gac ctg gct ctc att tcc cgg aat cgc ttt ggc ctt 1071 Gly Ala Ala Tyr Asp Leu Ala Leu Ile Ser Arg Asn Arg Phe Gly Leu 320 325 330 ggc ccc aac cag aca tgg cac ctt cct gcc tat aac cat tca gga cca 1119 Gly Pro Asn Gln Thr Trp His Leu Pro Ala Tyr Asn His Ser Gly Pro 335 340 345 ggg act ctg aat atc agt gtt gga gcc aat agg acc acc atg cat tgg 1167 Gly Thr Leu Asn Ile Ser Val Gly Ala Asn Arg Thr Thr Met His Trp 350 355 360 cca gtc ctg gcc cag ggc ctg acg tac tgc att gag tgg cag ccc cag 1215 Pro Val Leu Ala Gln Gly Leu Thr Tyr Cys Ile Glu Trp Gln Pro Gln 365 370 375 380 ggc cag gat gac agc ctt gtc aac tgt acc ttg atg aca ccc aag gac 1263 Gly Gln Asp Asp Ser Leu Val Asn Cys Thr Leu Met Thr Pro Lys Asp 385 390 395 cag gac cct gct gga atg gca gcc tac agc tgg agc caa gca ctt ggg 1311 Gln Asp Pro Ala Gly Met Ala Ala Tyr Ser Trp Ser Gln Ala Leu Gly 400 405 410 gca atg ggg cag aag gtg tgt tat cgc atc acc atc ttt gcc tct gca 1359 Ala Met Gly Gln Lys Val Cys Tyr Arg Ile Thr Ile Phe Ala Ser Ala 415 420 425 cgt cca gag aag ccc acg tca tgg tcc aca gtc tca tca acc tac tac 1407 Arg Pro Glu Lys Pro Thr Ser Trp Ser Thr Val Ser Ser Thr Tyr Tyr 430 435 440 ttt gca ggc aat gcc tca gga gcc ggg agc cca cag cat gtg tcg gtg 1455 Phe Ala Gly Asn Ala Ser Gly Ala Gly Ser Pro Gln His Val Ser Val 445 450 455 460 aag aga ctc aac cag gat tcc gtg tct gtg gac tgg aca cca tct ctg 1503 Lys Arg Leu Asn Gln Asp Ser Val Ser Val Asp Trp Thr Pro Ser Leu 465 470 475 ctg agt gcc tgc ccc ggc gtc ctg aag gag tac att gtg cgc tgc cat 1551 Leu Ser Ala Cys Pro Gly Val Leu Lys Glu Tyr Ile Val Arg Cys His 480 485 490 gat gag gac agc gac caa gta ttc gag ctg cca gtg aag cca aca gag 1599 Asp Glu Asp Ser Asp Gln Val Phe Glu Leu Pro Val Lys Pro Thr Glu 495 500 505 acc cgg gtc acc ctt caa ggc ctg cgg gct ggc gca gcc tac atg gtg 1647 Thr Arg Val Thr Leu Gln Gly Leu Arg Ala Gly Ala Ala Tyr Met Val 510 515 520 cag gtt cga gca gac aca gcc aag tgg cag ggc tcc tgg agc cag ccc 1695 Gln Val Arg Ala Asp Thr Ala Lys Trp Gln Gly Ser Trp Ser Gln Pro 525 530 535 540 ctg cgc ttc agc atc gaa gtc cct gtt tct gag ttg cgc gat ttg tcc 1743 Leu Arg Phe Ser Ile Glu Val Pro Val Ser Glu Leu Arg Asp Leu Ser 545 550 555 atc ttc ctg gca tct ttg ggg agc ttc atg agc atc ctt ctc ctg gga 1791 Ile Phe Leu Ala Ser Leu Gly Ser Phe Met Ser Ile Leu Leu Leu Gly 560 565 570 gtc ttt ggg tac ctt ggc ttg aac agg gtt gta agg cat ctg tgc ccg 1839 Val Phe Gly Tyr Leu Gly Leu Asn Arg Val Val Arg His Leu Cys Pro 575 580 585 ccc ctg ccc aca cct tgt gcc agc act gcc gtt gag ttc tct gac agc 1887 Pro Leu Pro Thr Pro Cys Ala Ser Thr Ala Val Glu Phe Ser Asp Ser 590 595 600 cag ggg aag cag gtt tgg cag tgg acc agc cca gca gac ttc ccg gag 1935 Gln Gly Lys Gln Val Trp Gln Trp Thr Ser Pro Ala Asp Phe Pro Glu 605 610 615 620 gag gtg tcc ccc caa gaa gcc ctg gtg gtg aac atg tcc tgg gac aaa 1983 Glu Val Ser Pro Gln Glu Ala Leu Val Val Asn Met Ser Trp Asp Lys 625 630 635 ggc gag ggg gtt gac cca gac aca cct ggg cct ctc aag gag aag atg 2031 Gly Glu Gly Val Asp Pro Asp Thr Pro Gly Pro Leu Lys Glu Lys Met 640 645 650 gag ctg cct ctg gat gcc cct gag ccg gcc cta gac atg gag ctg ccc 2079 Glu Leu Pro Leu Asp Ala Pro Glu Pro Ala Leu Asp Met Glu Leu Pro 655 660 665 ttg gag cac agg agg cag gcg caa gga cac cct gag cct ggg act ctg 2127 Leu Glu His Arg Arg Gln Ala Gln Gly His Pro Glu Pro Gly Thr Leu 670 675 680 agg tct ggg tgg cac ggt ggt ctg gag ggc agc cct gcc cag aca gct 2175 Arg Ser Gly Trp His Gly Gly Leu Glu Gly Ser Pro Ala Gln Thr Ala 685 690 695 700 gga ctc ctg ctg ctc ctg gga gat cta agg cag act ccc agg ttt ggt 2223 Gly Leu Leu Leu Leu Leu Gly Asp Leu Arg Gln Thr Pro Arg Phe Gly 705 710 715 tcc cag gga gaa agg gga aca tct gcc tcc tct tac ata gag gaa gac 2271 Ser Gln Gly Glu Arg Gly Thr Ser Ala Ser Ser Tyr Ile Glu Glu Asp 720 725 730 tgatgattgc aatcacgatg gctggttatt aagcacttac tgcataccca gcctgtgccg 2331 cccagacact catgttatgc cctgaatccc cataactgcc ttgaggtatg aggctgtcct 2391 atttacctat ttctctgagt ctcaccgagg ctcagagagg gtgagtgagt gccttgccta 2451 tggctacaca gcaggtgcat aagtcgaacc taggcggagt gactccgacc ctggccactg 2511 cttccattca cacctactct gtcctgctgt cacacatcct gtggtgggaa ccctagaaac 2571 caccatgaaa tggaagctgc taccccaagc cagggcctgg cacccatggg gatagactag 2631 agggaggctc aaaggggttc atggtggtcc tgggctgggc atcctgtacc agaagcactg 2691 gatggggaat ctgggtgcac agcgtcctcg gccacatcgc agggtctcca ggcttggagg 2751 atctaggttt agggaaaaga agtggggctc aaagcattct gagggaggtg atagctcctc 2811 cctggcctca gtctccttct ctgtaaaatg ggcctgagga taatgttgtc ctcacagaga 2871 tgggggagat gtaaggctta ataaattttt cccctggagt tctcattgtg gctcagcaga 2931 aacgaatcca acaggaacca tgaggttgcg ggttcgaccc ctggccttgc tcagtgggtt 2991 aaggatctgg tgttgctgtg agcaagacac agctaggatc ctacattgct gtggctgtgg 3051 ctgtggccag cagttgtagc tccaattcga ccccaagcaa gggaacctcc acatgctgca 3111 ggtgtagccc taaaaaaaaa aaaa 3135 8 732 PRT Porcine sp. 8 Met Trp Gln Arg Val Thr Arg Leu Val Pro Leu Phe Leu Leu Leu Phe 1 5 10 15 Pro Arg Gln Gly Ala Glu Ala Cys Ser Thr Ser Arg Cys Cys Phe Gln 20 25 30 Asp Pro Pro Tyr Pro Asp Ala Asp Ser Gly Ser Ala Ser Gly Pro Arg 35 40 45 Asp Leu Asn Cys Tyr Arg Ile Phe Asn Arg Ala Gly Tyr Glu Cys Ser 50 55 60 Trp Glu Tyr Glu Gly Pro Thr Ala Gly Val Ser His Phe Leu Arg Cys 65 70 75 80 Cys Leu Lys Ser Gly Arg Cys Cys Tyr Phe Ala Thr Gly Ser Ala Thr 85 90 95 Thr Leu Gln Phe Ser Asp Gln Asp Gly Val Asp Val Leu Asn Ala Val 100 105 110 Thr Leu Trp Val Glu Ser Arg Ala Ala Asn Lys Thr Glu Lys Ser Pro 115 120 125 Met Val Thr Leu Asn Leu Tyr Ser Ser Val Lys Tyr Asp Pro Pro Pro 130 135 140 Gly Asn Ile Lys Val Ser Arg Ser Ala Gly Gln Leu His Met Glu Trp 145 150 155 160 Glu Thr Pro Thr Arg Gln Asp Gly Ala Glu Ile Gln Phe Arg His Arg 165 170 175 Thr Pro Gly Ser Pro Trp Met Leu Gly Asp Cys Gly Arg Gln Asp Asp 180 185 190 Asp Ala Gly Phe Glu Ser Cys Leu Cys Pro Leu Glu Met Asp Met Ala 195 200 205 Gln Glu Phe Gln Leu Arg Arg Arg Arg His Gln Gly Pro Gly Val Pro 210 215 220 Gly Gly Pro Trp Ser Ser Trp Ser Ser Ser Val Cys Ile Pro Pro Glu 225 230 235 240 Asn Pro Pro Gln Ala Lys Ala Asn Phe Ser Val Glu Gln Leu Arg Ser 245 250 255 Asp Gly Arg Arg Gln Val Thr Leu Asn Glu Gln Leu Pro Gln Leu Glu 260 265 270 Leu Pro Glu Gly Cys Leu Gly Asn Asn Ser Gly Val Glu Met Thr Tyr 275 280 285 His Val His Leu His Met Leu Ser Cys Pro Cys Lys Ala Lys Ala Thr 290 295 300 Arg Thr Leu Arg Leu Arg Lys Lys Leu Val Leu Ser Gly Ala Ala Tyr 305 310 315 320 Asp Leu Ala Leu Ile Ser Arg Asn Arg Phe Gly Leu Gly Pro Asn Gln 325 330 335 Thr Trp His Leu Pro Ala Tyr Asn His Ser Gly Pro Gly Thr Leu Asn 340 345 350 Ile Ser Val Gly Ala Asn Arg Thr Thr Met His Trp Pro Val Leu Ala 355 360 365 Gln Gly Leu Thr Tyr Cys Ile Glu Trp Gln Pro Gln Gly Gln Asp Asp 370 375 380 Ser Leu Val Asn Cys Thr Leu Met Thr Pro Lys Asp Gln Asp Pro Ala 385 390 395 400 Gly Met Ala Ala Tyr Ser Trp Ser Gln Ala Leu Gly Ala Met Gly Gln 405 410 415 Lys Val Cys Tyr Arg Ile Thr Ile Phe Ala Ser Ala Arg Pro Glu Lys 420 425 430 Pro Thr Ser Trp Ser Thr Val Ser Ser Thr Tyr Tyr Phe Ala Gly Asn 435 440 445 Ala Ser Gly Ala Gly Ser Pro Gln His Val Ser Val Lys Arg Leu Asn 450 455 460 Gln Asp Ser Val Ser Val Asp Trp Thr Pro Ser Leu Leu Ser Ala Cys 465 470 475 480 Pro Gly Val Leu Lys Glu Tyr Ile Val Arg Cys His Asp Glu Asp Ser 485 490 495 Asp Gln Val Phe Glu Leu Pro Val Lys Pro Thr Glu Thr Arg Val Thr 500 505 510 Leu Gln Gly Leu Arg Ala Gly Ala Ala Tyr Met Val Gln Val Arg Ala 515 520 525 Asp Thr Ala Lys Trp Gln Gly Ser Trp Ser Gln Pro Leu Arg Phe Ser 530 535 540 Ile Glu Val Pro Val Ser Glu Leu Arg Asp Leu Ser Ile Phe Leu Ala 545 550 555 560 Ser Leu Gly Ser Phe Met Ser Ile Leu Leu Leu Gly Val Phe Gly Tyr 565 570 575 Leu Gly Leu Asn Arg Val Val Arg His Leu Cys Pro Pro Leu Pro Thr 580 585 590 Pro Cys Ala Ser Thr Ala Val Glu Phe Ser Asp Ser Gln Gly Lys Gln 595 600 605 Val Trp Gln Trp Thr Ser Pro Ala Asp Phe Pro Glu Glu Val Ser Pro 610 615 620 Gln Glu Ala Leu Val Val Asn Met Ser Trp Asp Lys Gly Glu Gly Val 625 630 635 640 Asp Pro Asp Thr Pro Gly Pro Leu Lys Glu Lys Met Glu Leu Pro Leu 645 650 655 Asp Ala Pro Glu Pro Ala Leu Asp Met Glu Leu Pro Leu Glu His Arg 660 665 670 Arg Gln Ala Gln Gly His Pro Glu Pro Gly Thr Leu Arg Ser Gly Trp 675 680 685 His Gly Gly Leu Glu Gly Ser Pro Ala Gln Thr Ala Gly Leu Leu Leu 690 695 700 Leu Leu Gly Asp Leu Arg Gln Thr Pro Arg Phe Gly Ser Gln Gly Glu 705 710 715 720 Arg Gly Thr Ser Ala Ser Ser Tyr Ile Glu Glu Asp 725 730 9 2224 DNA Porcine sp. CDS (19)..(765) 9 gatctctgca aggaccgg atg tgg caa cga gtg acc agg ctg gtc ccc ctc 51 Met Trp Gln Arg Val Thr Arg Leu Val Pro Leu 1 5 10 ttc ctc ctc ctg ttc ccg agg cag ggt gct gaa gcc tgc agt acc agc 99 Phe Leu Leu Leu Phe Pro Arg Gln Gly Ala Glu Ala Cys Ser Thr Ser 15 20 25 agg tgc tgt ttc cag gac ccg cca tat ccg gat gca gac tca ggc tca 147 Arg Cys Cys Phe Gln Asp Pro Pro Tyr Pro Asp Ala Asp Ser Gly Ser 30 35 40 gct tct ggc ccc agg gac cta aac tgc tac cgg ata ttc aac aga gct 195 Ala Ser Gly Pro Arg Asp Leu Asn Cys Tyr Arg Ile Phe Asn Arg Ala 45 50 55 ggt tat gaa tgt tcc tgg gag tat gag ggc ccc aca gct ggg gtc agc 243 Gly Tyr Glu Cys Ser Trp Glu Tyr Glu Gly Pro Thr Ala Gly Val Ser 60 65 70 75 cac ttc ctg aga tgc tgc ctc aag tct ggg cgc tgt tgc tac ttt gcc 291 His Phe Leu Arg Cys Cys Leu Lys Ser Gly Arg Cys Cys Tyr Phe Ala 80 85 90 aca ggc tca gcc acc aca ctg cag ttt tcc gac cag gat ggc gta gac 339 Thr Gly Ser Ala Thr Thr Leu Gln Phe Ser Asp Gln Asp Gly Val Asp 95 100 105 gtg ctc aat gct gtc act ctc tgg gtg gaa tcc cgg gct gcc aac aag 387 Val Leu Asn Ala Val Thr Leu Trp Val Glu Ser Arg Ala Ala Asn Lys 110 115 120 acg gag aag tcc ccc atg gtt acc ttg aat ctc tac agc tct gtt aaa 435 Thr Glu Lys Ser Pro Met Val Thr Leu Asn Leu Tyr Ser Ser Val Lys 125 130 135 tac gac cct ccc cca gga aac atc aag gta tca agg tca gca ggg cag 483 Tyr Asp Pro Pro Pro Gly Asn Ile Lys Val Ser Arg Ser Ala Gly Gln 140 145 150 155 ctg cac atg gag tgg gag acc cca acc cgc cag gat ggt gct gag ata 531 Leu His Met Glu Trp Glu Thr Pro Thr Arg Gln Asp Gly Ala Glu Ile 160 165 170 cag ttc cga cac cgg aca cct gga agc ccg tgg atg ctg agt cat gcc 579 Gln Phe Arg His Arg Thr Pro Gly Ser Pro Trp Met Leu Ser His Ala 175 180 185 tat gcc ccc tgg aga tgg aca tgg ccc aag aat tcc agc tgc gac gac 627 Tyr Ala Pro Trp Arg Trp Thr Trp Pro Lys Asn Ser Ser Cys Asp Asp 190 195 200 gac gac acc agg ggc cag ggg tcc cag gag gcc cct gga gca gct gga 675 Asp Asp Thr Arg Gly Gln Gly Ser Gln Glu Ala Pro Gly Ala Ala Gly 205 210 215 gca gct ctg tgt gca tcc ccc ctg aaa acc ccc cac agg cca agg cga 723 Ala Ala Leu Cys Ala Ser Pro Leu Lys Thr Pro His Arg Pro Arg Arg 220 225 230 235 att tct cag tgg agc agc tcc gct cag atg gga gga ggc agg 765 Ile Ser Gln Trp Ser Ser Ser Ala Gln Met Gly Gly Gly Arg 240 245 tgaccttgaa tgagcagctg ccccagcttg agcttccgga aggctgcctt gggaacaact 825 ctggagtgga aatgacctac catgttcacc tgcacatgct gtcctgccct tgtaaggcca 885 aggccacaag gactctgcgc ctaaggaaaa agctcgtcct ctcaggcgct gcctatgacc 945 tggctctcat ttcccggaat cgctttggcc ttggccccaa ccagacatgg caccttcctg 1005 cctataacca ttcaggacca gggactctga atatcagtgt tggagccaat aggaccacca 1065 tgcattggcc agtcctggcc cagggcctga cgtactgcat tgagtggcag ccccagggcc 1125 aggatgacag ccttgtcaac tgtaccttga tgacacccaa ggaccaggac cctgctggaa 1185 tggcagccta cagctggagc caagcacttg gggcaatggg gcagaaggtg tgttatcgca 1245 tcaccatctt tgcctctgca cgtccagaga agcccacgtc atggtccaca gtctcatcaa 1305 cctactactt tgcaggcaat gcctcaggag ccgggagccc acagcatgtg tcggtgaaga 1365 gactcaacca ggattccgtg tctgtggact ggacaccatc tctgctgagt gcctgccccg 1425 gcgtcctgaa ggagtacatt gtgcgctgcc atgatgagga cagcgaccaa gtattcgagc 1485 tgccagtgaa gccaacagag acccgggtca cccttcaagg cctgcgggct ggcgcagcct 1545 acatggtgca ggttcgagca gacacagcca agtggcaggg ctcctggagc cagcccctgc 1605 gcttcagcat cgaagtccct gtttctgagt tgcgcgattt gtccatcttc ctggcatctt 1665 tggggagctt catgagcatc cttctcctgg gagtctttgg gtaccttggc ttgaacaggg 1725 ttgtaaggca tctgtgcccg cccctgccca caccttgtgc cagcactgcc gttgagttct 1785 ctgacagcca ggggaagcag gtttggcagt ggaccagccc agcagacttc ccggaggagg 1845 tgtcccccca agaagccctg gtggtgaaca tgtcctggga caaaggcgag ggggttgacc 1905 cagacacacc tgggcctctc aaggagaaga tggagctgcc tctggatgcc cctgagccgg 1965 ccctagacat ggagctgccc ttggagcaca ggaggcaggc gcaaggacac cctgagcctg 2025 ggactctgag gtctgggtgg cacggtggtc tggagggcag ccctgcccag acagctggac 2085 tcctgctgct cctgggagat ctaaggcaga ctcccaggtt tggttcccag ggagaaaggg 2145 gaacatctgc ctcctcttac atagaggaag actgatgatt gcaatcacga tggctggtta 2205 ttaagcactt actgcatac 2224 10 249 PRT Porcine sp. 10 Met Trp Gln Arg Val Thr Arg Leu Val Pro Leu Phe Leu Leu Leu Phe 1 5 10 15 Pro Arg Gln Gly Ala Glu Ala Cys Ser Thr Ser Arg Cys Cys Phe Gln 20 25 30 Asp Pro Pro Tyr Pro Asp Ala Asp Ser Gly Ser Ala Ser Gly Pro Arg 35 40 45 Asp Leu Asn Cys Tyr Arg Ile Phe Asn Arg Ala Gly Tyr Glu Cys Ser 50 55 60 Trp Glu Tyr Glu Gly Pro Thr Ala Gly Val Ser His Phe Leu Arg Cys 65 70 75 80 Cys Leu Lys Ser Gly Arg Cys Cys Tyr Phe Ala Thr Gly Ser Ala Thr 85 90 95 Thr Leu Gln Phe Ser Asp Gln Asp Gly Val Asp Val Leu Asn Ala Val 100 105 110 Thr Leu Trp Val Glu Ser Arg Ala Ala Asn Lys Thr Glu Lys Ser Pro 115 120 125 Met Val Thr Leu Asn Leu Tyr Ser Ser Val Lys Tyr Asp Pro Pro Pro 130 135 140 Gly Asn Ile Lys Val Ser Arg Ser Ala Gly Gln Leu His Met Glu Trp 145 150 155 160 Glu Thr Pro Thr Arg Gln Asp Gly Ala Glu Ile Gln Phe Arg His Arg 165 170 175 Thr Pro Gly Ser Pro Trp Met Leu Ser His Ala Tyr Ala Pro Trp Arg 180 185 190 Trp Thr Trp Pro Lys Asn Ser Ser Cys Asp Asp Asp Asp Thr Arg Gly 195 200 205 Gln Gly Ser Gln Glu Ala Pro Gly Ala Ala Gly Ala Ala Leu Cys Ala 210 215 220 Ser Pro Leu Lys Thr Pro His Arg Pro Arg Arg Ile Ser Gln Trp Ser 225 230 235 240 Ser Ser Ala Gln Met Gly Gly Gly Arg 245 11 4023 DNA Porcine sp. CDS (230)..(2812) 11 gaggggaaga ggattctcgc agaggaaatg aaaagtgcac ggtccccgga gcagcaaagg 60 gcatgtcttg ttcagggaac cgtctgaagg ccagtgtgaa tgacgcagag ctgggaaggc 120 aaaagattag agccagtgat ggtggcaaga caggagatgt gaagccaggt agtggccgag 180 agtccgatgt gggaaaatac agagaggtgt atcccagagt tgactgttc atg gca cga 238 Met Ala Arg 1 aca gtt tgc ggg tgc tca tgg gcc ctt att ttt atc atc atg tca ctc 286 Thr Val Cys Gly Cys Ser Trp Ala Leu Ile Phe Ile Ile Met Ser Leu 5 10 15 ttg gtt aag gca aaa ata gat gtg tgc aag aga ggg gat gtg acg gtg 334 Leu Val Lys Ala Lys Ile Asp Val Cys Lys Arg Gly Asp Val Thr Val 20 25 30 35 cag cct tcc cat gta att tca ctt gga tca gct gtc aac att tca tgc 382 Gln Pro Ser His Val Ile Ser Leu Gly Ser Ala Val Asn Ile Ser Cys 40 45 50 tct ttg aag ccc agg caa ggc tgc tta caa gtc tcc agt tta aac aag 430 Ser Leu Lys Pro Arg Gln Gly Cys Leu Gln Val Ser Ser Leu Asn Lys 55 60 65 cta atc ctc tac agg ttc cac agg aga atc cac ttt cag cgt ggt cac 478 Leu Ile Leu Tyr Arg Phe His Arg Arg Ile His Phe Gln Arg Gly His 70 75 80 tcc ctc agt tct caa gtc aca ggt ctt ccc ctg ggt acc acc ctg ttc 526 Ser Leu Ser Ser Gln Val Thr Gly Leu Pro Leu Gly Thr Thr Leu Phe 85 90 95 gtc tgc aaa ctg gcc tgt agc agt aag gag gag att cga ata tgt ggg 574 Val Cys Lys Leu Ala Cys Ser Ser Lys Glu Glu Ile Arg Ile Cys Gly 100 105 110 115 gcc gag atc tcg gtt ggt gtt gtt cca gaa cag cct caa aac gta tct 622 Ala Glu Ile Ser Val Gly Val Val Pro Glu Gln Pro Gln Asn Val Ser 120 125 130 tgt atg cag aag gga gaa cgt ggg aca gtg gcc tgc agc tgg gac aga 670 Cys Met Gln Lys Gly Glu Arg Gly Thr Val Ala Cys Ser Trp Asp Arg 135 140 145 gga cga gat acc cat cta tat act gca tat act tta caa tta aat gga 718 Gly Arg Asp Thr His Leu Tyr Thr Ala Tyr Thr Leu Gln Leu Asn Gly 150 155 160 cca aaa aat tta act tgg cag aag caa tgt agc gat tat tat tgt gac 766 Pro Lys Asn Leu Thr Trp Gln Lys Gln Cys Ser Asp Tyr Tyr Cys Asp 165 170 175 tct ttg gac ctt gga atc aac cta ccc cct gaa tca cct gaa tct agt 814 Ser Leu Asp Leu Gly Ile Asn Leu Pro Pro Glu Ser Pro Glu Ser Ser 180 185 190 195 tac aca gcc cag gtt act gcc atc aat agt cta ggg act gcg tct tca 862 Tyr Thr Ala Gln Val Thr Ala Ile Asn Ser Leu Gly Thr Ala Ser Ser 200 205 210 ttt cca tgc aca ttc aca ttg ttg gat gta gtg agg cct ctt cct ccg 910 Phe Pro Cys Thr Phe Thr Leu Leu Asp Val Val Arg Pro Leu Pro Pro 215 220 225 tgg gac atc aga atc aaa tgt gta aat gcc tcc gtg agc aca tgt acc 958 Trp Asp Ile Arg Ile Lys Cys Val Asn Ala Ser Val Ser Thr Cys Thr 230 235 240 ctg caa tgg aga gat gag ggg cta gtg ctg ctt aat cga ctc aga tac 1006 Leu Gln Trp Arg Asp Glu Gly Leu Val Leu Leu Asn Arg Leu Arg Tyr 245 250 255 cgg cct gtt tac agc aga tcc tgg aat atg gtt aat gct aca aat gcc 1054 Arg Pro Val Tyr Ser Arg Ser Trp Asn Met Val Asn Ala Thr Asn Ala 260 265 270 275 aaa gga aga cat gat ttg gtg gat ctg aaa ccc ttt aca gaa tat gaa 1102 Lys Gly Arg His Asp Leu Val Asp Leu Lys Pro Phe Thr Glu Tyr Glu 280 285 290 ttt cag att tcc tct aag cca cat ctt cag aag ggt aga tgg agt gat 1150 Phe Gln Ile Ser Ser Lys Pro His Leu Gln Lys Gly Arg Trp Ser Asp 295 300 305 tgg agt gaa tca ttg aga act caa act cca gag aaa gag cct aca ggg 1198 Trp Ser Glu Ser Leu Arg Thr Gln Thr Pro Glu Lys Glu Pro Thr Gly 310 315 320 atg tta gac gtc tgg tac atg aaa cag cac att gac tac aaa aga caa 1246 Met Leu Asp Val Trp Tyr Met Lys Gln His Ile Asp Tyr Lys Arg Gln 325 330 335 cag att tat ctt ttc tgg aag aat ctg agt cta tca gag gca aga gga 1294 Gln Ile Tyr Leu Phe Trp Lys Asn Leu Ser Leu Ser Glu Ala Arg Gly 340 345 350 355 aaa atc ctc cac tat caa gtg acc ttg cag gag gtc gca gag ggg aat 1342 Lys Ile Leu His Tyr Gln Val Thr Leu Gln Glu Val Ala Glu Gly Asn 360 365 370 gcc aca ctg cag aat atc act gaa cgc aac tcc tgg acc tgg acc ata 1390 Ala Thr Leu Gln Asn Ile Thr Glu Arg Asn Ser Trp Thr Trp Thr Ile 375 380 385 ccc aga act ggc atc tgg gct gcg gcc gtg tct gca gct aac tcc aaa 1438 Pro Arg Thr Gly Ile Trp Ala Ala Ala Val Ser Ala Ala Asn Ser Lys 390 395 400 ggc agt tcc ctg ccc act cgt att aac ata gca gac ctg tgc gga gca 1486 Gly Ser Ser Leu Pro Thr Arg Ile Asn Ile Ala Asp Leu Cys Gly Ala 405 410 415 ggg ttg ctg gct cct cag cag gtt tct gca aac cca gag ggc tcg gac 1534 Gly Leu Leu Ala Pro Gln Gln Val Ser Ala Asn Pro Glu Gly Ser Asp 420 425 430 435 aac ctc ctg gtg aag tgg acg tct ccg ggg gaa ggt gcc act gct gtg 1582 Asn Leu Leu Val Lys Trp Thr Ser Pro Gly Glu Gly Ala Thr Ala Val 440 445 450 cag gag tat gtg gtg gag tgg agg gag ctc cat ctg agg ggt ggc atg 1630 Gln Glu Tyr Val Val Glu Trp Arg Glu Leu His Leu Arg Gly Gly Met 455 460 465 cag ccc cct cta agc tgg ctg cgg agt ccc ccc tac aac acg tcc acc 1678 Gln Pro Pro Leu Ser Trp Leu Arg Ser Pro Pro Tyr Asn Thr Ser Thr 470 475 480 ctc atc tca gac aac ata aaa ccc tac atc tgt tat gaa atc cga gtg 1726 Leu Ile Ser Asp Asn Ile Lys Pro Tyr Ile Cys Tyr Glu Ile Arg Val 485 490 495 cac gca ctt tca ggg gac cag gga gga tgc agc tcc atc cgg ggt gac 1774 His Ala Leu Ser Gly Asp Gln Gly Gly Cys Ser Ser Ile Arg Gly Asp 500 505 510 515 ttg aag cat aaa gca cca ctg agt ggc ccc cac att aat gcc atc tca 1822 Leu Lys His Lys Ala Pro Leu Ser Gly Pro His Ile Asn Ala Ile Ser 520 525 530 gag gaa aag ggg agc att tta att tca tgg gac gaa att cca gcc cag 1870 Glu Glu Lys Gly Ser Ile Leu Ile Ser Trp Asp Glu Ile Pro Ala Gln 535 540 545 gag caa atg ggc tgc atc ctc cat tac agg atc tat tgg aag gaa cgg 1918 Glu Gln Met Gly Cys Ile Leu His Tyr Arg Ile Tyr Trp Lys Glu Arg 550 555 560 gac tcc gat tcc cag cct cag ctc tgt gaa att ccc tat aga gtc tcc 1966 Asp Ser Asp Ser Gln Pro Gln Leu Cys Glu Ile Pro Tyr Arg Val Ser 565 570 575 ccc aaa tca cat ccc atc aat agc ctg cag ccc aga gtg aca tac gtc 2014 Pro Lys Ser His Pro Ile Asn Ser Leu Gln Pro Arg Val Thr Tyr Val 580 585 590 595 ctg tgg atg aca gct ctg aca gct gct ggc gaa agc ccc caa gga aat 2062 Leu Trp Met Thr Ala Leu Thr Ala Ala Gly Glu Ser Pro Gln Gly Asn 600 605 610 gag agg gaa ttt tgt ctg caa ggt aaa gcc aat tgg agc aca ttt gta 2110 Glu Arg Glu Phe Cys Leu Gln Gly Lys Ala Asn Trp Ser Thr Phe Val 615 620 625 gca cca agc att tgc att gct gtc atc aca gtg ggc gtt ttc tca atg 2158 Ala Pro Ser Ile Cys Ile Ala Val Ile Thr Val Gly Val Phe Ser Met 630 635 640 cgt tgc ttc cgg caa aag gta ttt gtt ctc ctt ttg gcc ctc aga cct 2206 Arg Cys Phe Arg Gln Lys Val Phe Val Leu Leu Leu Ala Leu Arg Pro 645 650 655 cag tgg tgt agc aga gaa att cca gac cca gcc aat agc act tgg gcc 2254 Gln Trp Cys Ser Arg Glu Ile Pro Asp Pro Ala Asn Ser Thr Trp Ala 660 665 670 675 aag aaa tat ccc att gtg gag gag aag aaa cag ctg tcc ctg gat agg 2302 Lys Lys Tyr Pro Ile Val Glu Glu Lys Lys Gln Leu Ser Leu Asp Arg 680 685 690 ctc ctg gca gac tgg ccc act cct gaa gaa cct gag ccc ctg gtc atc 2350 Leu Leu Ala Asp Trp Pro Thr Pro Glu Glu Pro Glu Pro Leu Val Ile 695 700 705 aat gaa gtc ctt cct caa gtg act cca gtc ttc aga cgc ccc cat cat 2398 Asn Glu Val Leu Pro Gln Val Thr Pro Val Phe Arg Arg Pro His His 710 715 720 ccc aac tgg cca gga aag gga caa agg ctc caa ggt cac cac gcc tct 2446 Pro Asn Trp Pro Gly Lys Gly Gln Arg Leu Gln Gly His His Ala Ser 725 730 735 gag gaa gac aca ggg tcc agt gcc tca agt cca cca cct cca agg gct 2494 Glu Glu Asp Thr Gly Ser Ser Ala Ser Ser Pro Pro Pro Pro Arg Ala 740 745 750 755 ctc aca gca gag aca gga ccc gca gtg gat ctg tac aag gtg ctg ggg 2542 Leu Thr Ala Glu Thr Gly Pro Ala Val Asp Leu Tyr Lys Val Leu Gly 760 765 770 agc cga cgc cct gac tca aag ccg gga aac cca gtc agc cac ttg acg 2590 Ser Arg Arg Pro Asp Ser Lys Pro Gly Asn Pro Val Ser His Leu Thr 775 780 785 gtc ctc ccc gtg gac tac ctg cct acc cat gag ggt tac tta ccc tcc 2638 Val Leu Pro Val Asp Tyr Leu Pro Thr His Glu Gly Tyr Leu Pro Ser 790 795 800 aac atg gat tat ctc cct tca cat gaa gct ccc ata aca gac tct ctg 2686 Asn Met Asp Tyr Leu Pro Ser His Glu Ala Pro Ile Thr Asp Ser Leu 805 810 815 gaa gaa ctg cct cag cac atc tct ctt tcc gtt ttc ccc tca aat tcc 2734 Glu Glu Leu Pro Gln His Ile Ser Leu Ser Val Phe Pro Ser Asn Ser 820 825 830 835 ctt cac ccg ctg acc ttc tcc tgt ggt gag aag ttg act ctg gat cag 2782 Leu His Pro Leu Thr Phe Ser Cys Gly Glu Lys Leu Thr Leu Asp Gln 840 845 850 ttg aag atg ggg tgt ggc tct ctc atg ctc tgagtggtga agcgtgatgt 2832 Leu Lys Met Gly Cys Gly Ser Leu Met Leu 855 860 ttggaaagtc agtgtggccc cagccagcaa agcatggcct gtctacccag cctcctgctc 2892 cgggatcatc gcccctgggc gctaccatta ggtctggttc caactagagg gcaggcaaac 2952 cagcttaggg caaatctcag gaactgagag ttggctgtaa cccagatacc tcacactgcc 3012 ttctcctgag cctcgagact acgtccccca ctctgtggac ctggagactc caacttgggt 3072 acctgcaact ctcttggcta tgttggccag aaagggacaa ggcaagagta gaaaccaaaa 3132 ctcgttgaat cagagctgca gctaccagcc tacaccagcc acagcaacac aggatccgag 3192 ccacctgtgc cacctacacc atagctcacg gcaacatcgg atccttaacc cactgagtga 3252 ggccaggaat tgaacctgca tcctcacaga caccatgtca ggttcttaac ccactgagcc 3312 acaacgggaa ctccacattt tttttttttt tagggctgca cctgtggcaa tatggaagtt 3372 tccagcctag gggtcaaatt ggagctacag ctaccagcct gtgccacagc tacagcaatg 3432 ccagatctga gccacaagtg cgacctacac cacagctcgt ggcaacacca gatccttaac 3492 ccactgagca aggccaagga ttgaacccac atcctcatgg acactagtcg gatttgcttc 3552 cactaagcca ccaagggaac tcccagattt ttaaaaataa taaattctac agtactacat 3612 gatccattgt tggatgaacc catggataca gaagcacaga tacagaaggc caactaaaag 3672 ttatacgctg attttcaact gtacggggag ctggtgctcc taaccccatg ttgttcaagg 3732 gtcatcgtat aaagcagcta ctacaaacca ggtcttatgt ctagatgaga actgtccacc 3792 acatcggcca ttgaccactt tggaatatac ctctagtatg actgaggagc tggtttttat 3852 tgtaattaaa atagtcacat atggctcttg gctactatac tggagagcac aactctaaat 3912 gactattaag acaatatgaa gaaaatttca tttttgtcta attgcaatta aaagaagatg 3972 ttaaaatatc caaatgaaaa taaaattaat actttcacaa aaaaaaaaaa a 4023 12 861 PRT Porcine sp. 12 Met Ala Arg Thr Val Cys Gly Cys Ser Trp Ala Leu Ile Phe Ile Ile 1 5 10 15 Met Ser Leu Leu Val Lys Ala Lys Ile Asp Val Cys Lys Arg Gly Asp 20 25 30 Val Thr Val Gln Pro Ser His Val Ile Ser Leu Gly Ser Ala Val Asn 35 40 45 Ile Ser Cys Ser Leu Lys Pro Arg Gln Gly Cys Leu Gln Val Ser Ser 50 55 60 Leu Asn Lys Leu Ile Leu Tyr Arg Phe His Arg Arg Ile His Phe Gln 65 70 75 80 Arg Gly His Ser Leu Ser Ser Gln Val Thr Gly Leu Pro Leu Gly Thr 85 90 95 Thr Leu Phe Val Cys Lys Leu Ala Cys Ser Ser Lys Glu Glu Ile Arg 100 105 110 Ile Cys Gly Ala Glu Ile Ser Val Gly Val Val Pro Glu Gln Pro Gln 115 120 125 Asn Val Ser Cys Met Gln Lys Gly Glu Arg Gly Thr Val Ala Cys Ser 130 135 140 Trp Asp Arg Gly Arg Asp Thr His Leu Tyr Thr Ala Tyr Thr Leu Gln 145 150 155 160 Leu Asn Gly Pro Lys Asn Leu Thr Trp Gln Lys Gln Cys Ser Asp Tyr 165 170 175 Tyr Cys Asp Ser Leu Asp Leu Gly Ile Asn Leu Pro Pro Glu Ser Pro 180 185 190 Glu Ser Ser Tyr Thr Ala Gln Val Thr Ala Ile Asn Ser Leu Gly Thr 195 200 205 Ala Ser Ser Phe Pro Cys Thr Phe Thr Leu Leu Asp Val Val Arg Pro 210 215 220 Leu Pro Pro Trp Asp Ile Arg Ile Lys Cys Val Asn Ala Ser Val Ser 225 230 235 240 Thr Cys Thr Leu Gln Trp Arg Asp Glu Gly Leu Val Leu Leu Asn Arg 245 250 255 Leu Arg Tyr Arg Pro Val Tyr Ser Arg Ser Trp Asn Met Val Asn Ala 260 265 270 Thr Asn Ala Lys Gly Arg His Asp Leu Val Asp Leu Lys Pro Phe Thr 275 280 285 Glu Tyr Glu Phe Gln Ile Ser Ser Lys Pro His Leu Gln Lys Gly Arg 290 295 300 Trp Ser Asp Trp Ser Glu Ser Leu Arg Thr Gln Thr Pro Glu Lys Glu 305 310 315 320 Pro Thr Gly Met Leu Asp Val Trp Tyr Met Lys Gln His Ile Asp Tyr 325 330 335 Lys Arg Gln Gln Ile Tyr Leu Phe Trp Lys Asn Leu Ser Leu Ser Glu 340 345 350 Ala Arg Gly Lys Ile Leu His Tyr Gln Val Thr Leu Gln Glu Val Ala 355 360 365 Glu Gly Asn Ala Thr Leu Gln Asn Ile Thr Glu Arg Asn Ser Trp Thr 370 375 380 Trp Thr Ile Pro Arg Thr Gly Ile Trp Ala Ala Ala Val Ser Ala Ala 385 390 395 400 Asn Ser Lys Gly Ser Ser Leu Pro Thr Arg Ile Asn Ile Ala Asp Leu 405 410 415 Cys Gly Ala Gly Leu Leu Ala Pro Gln Gln Val Ser Ala Asn Pro Glu 420 425 430 Gly Ser Asp Asn Leu Leu Val Lys Trp Thr Ser Pro Gly Glu Gly Ala 435 440 445 Thr Ala Val Gln Glu Tyr Val Val Glu Trp Arg Glu Leu His Leu Arg 450 455 460 Gly Gly Met Gln Pro Pro Leu Ser Trp Leu Arg Ser Pro Pro Tyr Asn 465 470 475 480 Thr Ser Thr Leu Ile Ser Asp Asn Ile Lys Pro Tyr Ile Cys Tyr Glu 485 490 495 Ile Arg Val His Ala Leu Ser Gly Asp Gln Gly Gly Cys Ser Ser Ile 500 505 510 Arg Gly Asp Leu Lys His Lys Ala Pro Leu Ser Gly Pro His Ile Asn 515 520 525 Ala Ile Ser Glu Glu Lys Gly Ser Ile Leu Ile Ser Trp Asp Glu Ile 530 535 540 Pro Ala Gln Glu Gln Met Gly Cys Ile Leu His Tyr Arg Ile Tyr Trp 545 550 555 560 Lys Glu Arg Asp Ser Asp Ser Gln Pro Gln Leu Cys Glu Ile Pro Tyr 565 570 575 Arg Val Ser Pro Lys Ser His Pro Ile Asn Ser Leu Gln Pro Arg Val 580 585 590 Thr Tyr Val Leu Trp Met Thr Ala Leu Thr Ala Ala Gly Glu Ser Pro 595 600 605 Gln Gly Asn Glu Arg Glu Phe Cys Leu Gln Gly Lys Ala Asn Trp Ser 610 615 620 Thr Phe Val Ala Pro Ser Ile Cys Ile Ala Val Ile Thr Val Gly Val 625 630 635 640 Phe Ser Met Arg Cys Phe Arg Gln Lys Val Phe Val Leu Leu Leu Ala 645 650 655 Leu Arg Pro Gln Trp Cys Ser Arg Glu Ile Pro Asp Pro Ala Asn Ser 660 665 670 Thr Trp Ala Lys Lys Tyr Pro Ile Val Glu Glu Lys Lys Gln Leu Ser 675 680 685 Leu Asp Arg Leu Leu Ala Asp Trp Pro Thr Pro Glu Glu Pro Glu Pro 690 695 700 Leu Val Ile Asn Glu Val Leu Pro Gln Val Thr Pro Val Phe Arg Arg 705 710 715 720 Pro His His Pro Asn Trp Pro Gly Lys Gly Gln Arg Leu Gln Gly His 725 730 735 His Ala Ser Glu Glu Asp Thr Gly Ser Ser Ala Ser Ser Pro Pro Pro 740 745 750 Pro Arg Ala Leu Thr Ala Glu Thr Gly Pro Ala Val Asp Leu Tyr Lys 755 760 765 Val Leu Gly Ser Arg Arg Pro Asp Ser Lys Pro Gly Asn Pro Val Ser 770 775 780 His Leu Thr Val Leu Pro Val Asp Tyr Leu Pro Thr His Glu Gly Tyr 785 790 795 800 Leu Pro Ser Asn Met Asp Tyr Leu Pro Ser His Glu Ala Pro Ile Thr 805 810 815 Asp Ser Leu Glu Glu Leu Pro Gln His Ile Ser Leu Ser Val Phe Pro 820 825 830 Ser Asn Ser Leu His Pro Leu Thr Phe Ser Cys Gly Glu Lys Leu Thr 835 840 845 Leu Asp Gln Leu Lys Met Gly Cys Gly Ser Leu Met Leu 850 855 860 

We claim:
 1. A porcine IL-12 receptor encoded by the cDNA molecule subunits of the porcine beta-1 chain and porcine beta-2 chain.
 2. A cDNA molecule encoding the porcine beta-1 chain of the porcine interleulkin-12 receptor, said cDNA molecule having a nucleotide sequence as shown in FIG.
 1. 3. A cDNA molecule encoding the porcine beta-2 chain of the porcine interleukin-12 receptor, said cDNA sequence having a nucleotide sequence as shown in FIG.
 5. 4. A method of identifying pigs capable of developing a strong cellular immune response to a given pathogen, said method comprising: (a) specifying a given pathogen; and (b) selecting pigs with allelic variants of an IL-12 receptor that confer on the pigs a superior ability compared to pigs without the variants, to develop a strong cellular response upon infection or vaccination against the pathogen.
 5. A molecule with an amino acid sequence deduced from the cDNA sequence of claim 1, said amino acid sequence as shown in FIG.
 1. 6. A molecule with an amino acid sequence deduced from the cDNA sequence of claim 2, said amino acid sequence as shown in FIG.
 5. 7. A method for improving the cellular immune response of pig herds, said method comprising: (a) selecting pigs with allelic variants of an IL-12 receptor that confer on the pigs a superior ability compared to pigs without the variants, to develop a strong cellular response upon infection or vaccination against the pathogen; and (b) breeding the selected pigs according to methods known to those of skill in the art of pig breeding.
 8. A molecule with an amino acid sequence deduced from the cDNA sequence of claim 3, said amino acid sequence having an allelic variant at position 1254 that results in a serine at amino acid position #342 as shown in FIG.
 5. 9. The molecule of claim 8 wherein the variant result from a nucleotide change at position 1254 from A to C.
 10. The molecule of claim 8 further characterized as having associate as a high IFN-g response or cell mediated immunity to an infectious agent in a pig.
 11. The molecule of claim 8 further characterized as associated with a high IFN-g response or cell mediated immunity to the infectious agent PRRSV in a pig.
 12. The molecule of claim 8 further characterized as associated with a high immune response to microorganims.
 13. The molecule of claim 8 further characterized as associated with a high immune response to viruses. 